α-Amine Ligation Profiling Informing N-terminal modification Enzyme selection
Subtiligase binds the first two N-terminal amino acids of its peptide substrates. To choose the best subtiligase variant for the protein or peptide substrate you wish to label, enter the first two amino acids of its sequence. For example, if the protein begins with alanine-phenylalanine, enter AF. Note that subtiligase labeling efficiency will also depend on N-terminal accessibility.