α-Amine Ligation Profiling Informing N-terminal modification Enzyme selection
Proteomic Identification of Ligation Sites (PILS) enables comprehensive profiling of peptide ligase (or other N-terminal modification reaction) specificity by quantifying enrichment of each sequence compared to the input peptide library. To analyze enrichment for a PILS dataset, enter a list of enriched sequences, then choose to enter a list of reference sequences or to use a Wells Lab reference dataset. Clicking 'Submit' will generated a PILS specificity heatmap and a csv file listing the calculated enrichment score for each dipeptide sequence.
Download a sample PILS dataset by clicking here.