α-Amine Ligation Profiling Informing N-terminal modification Enzyme selection
Subtiligase has been used as a tool for global enrichment and sequencing of neo-N-termini generated by proteolytic cleavage events. Application of subtiligase variant cocktails can improve sequence coverage in these N-terminomics experiments. To create a custom subtiligase cocktail for your application, enter a list of dipeptide sequences for which you would like to achieve maximum coverage.
Download a list of all 400 dipeptide sequences by clicking here.